UV Colorimetric Method

Monoreagent

Quantitative determination of Phosphorous on serum and urine.

Phosphorus can be found in high concentrations in the organism (650g) in many different chemical forms. Its importance is related to different metabolisms: bones, lipids, glycols. Around 80% of phosphorus  can be found in skeletal tissue and the rest is in the muscular tissue and organic liquids. Phosphorus is absorbed with foods and the majority is eliminated through urine. Its metabolism is strictly linked with the metabolism of calcium and it is regulated by the PHT (para thyroid hormone), by vitamin D and by calcitonin. An increase in phosphorus leads to a decrease of calcium levels. The fraction that is typically determined in a clinical laboratory is the inorganic part, found in serum. Low phosphorus levels can indicate rickets, Fanconi syndrome and hyperparathyroidism. An increase in phosphorus levels can lead back to vitamin D intoxication, hypoparathyroidism and renal failure with reduced glomerular filtration