ColorimetricFerrozine Method

Bireagent

Quantitative determination of iron on serum.

 The majority of iron that transits in plasma derives from the destruction of red blood cells. The rest derives from reserve iron and, only in a small percentage, from the individual’s diet. The dosage of total iron in serum is the determination of the quantity of plasmatic transport iron, linked to transferrin. Iron deficit causes microcytic anemia: low values of haemoglobin and of average corpuscular volume of red blood cells should always lead to investigation on iron exchange. On the contrary, high levels of total iron are caused by massive blood transfusions,  hemochromatosis, aplastic and hemolytic anemia, thalassemia, lead poisoning, vitamin B6 deficiency, excessive iron therapy.