Cytochemical staining on blood or bone marrow smears

The reaction detects several intracellular lipids, including neutral fats, phospholipids and sterols, as fats in general can dissolve some stains which are removed from their alcoholic solution. After staining, black color lipidic granules appear in all granulocytes with progressive increase in positive reaction from myeloblast to mature granulocyte. Lipidic granules are also present in Auer’s bodies and may sometimes appear as a fine cytoplasmatic dispersion in monocytes. Lymphocytes, lymphoblasts and erythroblasts are negative to Sudan Black B. The kit is used to identify the origin of monocytic cells and thus to distinguish between granulocytic and monocytic leukemia.